Download e-book for kindle: A Laboratory Course in Tissue Engineering by Melissa Kurtis Micou
By Melissa Kurtis Micou
Filling the necessity for a lab textbook during this speedily growing to be box, A Laboratory path in Tissue Engineering helps scholars boost hands-on event. The publication includes fifteen standalone experiments in response to either vintage tissue-engineering techniques and up to date advances within the box. Experiments surround a collection of extensively acceptable recommendations: mobilephone tradition, microscopy, histology, immunohistochemistry, mechanical trying out, tender lithography, and customary biochemical assays. as well as educating those particular recommendations, the experiments emphasize engineering research, mathematical modeling, and statistical experimental layout.
A reliable starting place in Tissue Engineering—and communique Skills
Each test comprises history info, studying pursuits, an outline, protection notes, an inventory of fabrics, recipes, equipment, pre- and postlab questions, and references. Emphasizing the significance for engineering scholars to improve powerful communique abilities, each one test additionally includes a information research and reporting part that provides a framework for succinctly documenting key effects. A separate bankruptcy offers directions for reporting ends up in the shape of a technical document, magazine article, prolonged summary, summary, or technical poster.
Customize Your classes with greater than a Semester’s worthy of Experiments
The publication is a handy resource of tutorial fabric applicable for undergraduate or graduate scholars with basic wisdom of engineering and telephone biology. the entire experiments were generally validated to enhance the possibility of winning facts assortment. furthermore, to reduce lab expenses, the experiments make wide use of apparatus as a rule present in laboratories built for tissue tradition. A suggestions handbook, on hand with qualifying path adoption, contains solutions to pre- and postlab questions, urged apparatus providers and product numbers, and different assets to assist plan a brand new tissue engineering course.
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Extra resources for A Laboratory Course in Tissue Engineering
Place the 15 mL tube opposite an appropriate counterbalance in a tabletop centrifuge. Centrifuge for 5 min at 250–500 g. When centrifugation is complete, a small white pellet should be visible at the bottom of the tube; these are the cells. Aseptically move the tube back into the BSC. 9. Loosen the cap of the 15 mL tube. Open the lid of the canister that holds the Pasteur pipettes; hold the canister horizontally and gently shake the pipettes out a bit so that you can select one pipette without touching the others.
Label the flask to indicate the cell line, passage number, date, and your name. You can write directly on the flask since it is disposable. 14. Transfer the flask into a 37°C CO2 incubator. 15. Discard the cryogenic vial and the 15 mL tube into the biohazard waste. Discard the Pasteur pipette into the biohazard sharps waste. Store the complete DMEM at 4°C. Wipe down the BSC with 70% ethanol. 6 Refeeding Cell Cultures/Changing Media As cultured cells proliferate, nutrients in the media are gradually depleted and waste accumulates.
If the protocol you are using is ambiguous, ask your instructor for clarification. Different notations are used to express the concentrations of solutions; some notations should be familiar to you from general chemistry lab but others are probably unfamiliar. The following sections summarize these common methods for expressing solution concentrations. An excellent reference for additional information is Lab Math: A Handbook of Measurements, Calculations, and Other Quantitative Skills for Use at the Bench (Adams 2003).
A Laboratory Course in Tissue Engineering by Melissa Kurtis Micou